ccl2  (Boster Bio)

Journal: Cell Communication and Signaling : CCS

Article Title: Fusobacterium nucleatum -reprogrammed adipocytes promote tumor cisplatin resistance through the CCL2-CCR2 axis in the necrotic metastatic neck nodes of head and neck carcinoma

doi: 10.1186/s12964-025-02550-z

Figure Lengend Snippet: F. nucleatum -reprogrammed adipocytes facilitate tumor cisplatin-resistance through activation of CREB/HSL and secretion of CCL2. A Western blot analysis of phosphorylation level of CREB/HSL in adipocytes and F. nucleatum -cocultured adipocytes ( n = 3). B Western blot analysis of lipophagy-related genes in adipocytes and F. nucleatum -cocultured adipocytes ( n = 3). C PCA plot showed the overall differences in transcriptional profile of adipocytes and F. nucleatum -cocultured adipocytes. D The circle heatmap of differential genes in adipocytes and F. nucleatum -cocultured adipocytes. E KEGG pathway enrichment. F MA plot of differential genes with cytokine-related genes highlighted. G ELISA analysis of CCL2 content in the supernatant of adipocytes and F. nucleatum -cocultured adipocytes ( n = 3). H Changes in CCL2 expression in inguinal adipose tissue following F. nucleatum infection. AdiCM: adipocyte supernatant. FN: Fusobacterium nucleatum . Adi: adipocytes. Data are presented as means ± SEM. * P < 0.05,‌** P < 0.01, ‌*** P < 0.001, **** P < 0.0001, ns: no statistical significance

Article Snippet: The ELISA quantitation of CCL2 was performed using Mouse MCP-1/CCL2 ELISA Kit (Boster Biological Technology, China).

Techniques: Activation Assay, Western Blot, Phospho-proteomics, Enzyme-linked Immunosorbent Assay, Expressing, Infection

Journal: Cell Communication and Signaling : CCS

Article Title: HOXC6 promotes the metastasis of MSI-H CRC by interacting with M2 macrophages and inducing effector T cell exhaustion

doi: 10.1186/s12964-025-02167-2

Figure Lengend Snippet: HOXC6 was correlated with enhanced cytokine interaction and TME reshuffling. A Volcano plot of DEGs identified between the low and high HOXC6 expression groups using TCGA samples. B GO enrichment of DEGs in the high HOXC6 expression group using TCGA samples. C KEGG pathway enrichment of DEGs in the HOXC6 high-expression group using TCGA samples. D Tumor purity, leukocyte fraction, and M2 macrophage infiltration were compared between the HOXC6 high- and low-expression groups. E Higher M2 macrophage infiltration was observed in the high HOXC6 expression group than in the low HOXC6 expression group in the ZUCI cohort. F Relative mRNA expression of CCL2 in the HOXC6-OE and siHOXC6 groups compared to the NC group in HCT116 cells. G ELISA detection of CCL2 concentrations in the HOXC6-OE and siHOXC6 groups compared to the NC group using HCT116 cell supernatants. H Representative images of samples with high HOXC6 expression and high M2 macrophage infiltration. I Correlation between HOXC6 expression and M2-TAM infiltration in 53 RCC samples from the ZUCI cohort. *: P < 0.05; **: P < 0.01; ***: P < 0.001

Article Snippet: Detection of secreted human CCL2, IFN-γ, and TNF-α were performed using h.MCP-1 ELISA kit (BOSTER, Wuhan, China, Lot: EK0441), IFN-γ ELISA kit (BOSTER, Wuhan, China, Lot: EK0373), and TNF-α ELISA kit (Absin, Shanghai, China, Lot: abs551818) according to the manufacturers’ instructions.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay

Journal: Cell Communication and Signaling : CCS

Article Title: HOXC6 promotes the metastasis of MSI-H CRC by interacting with M2 macrophages and inducing effector T cell exhaustion

doi: 10.1186/s12964-025-02167-2

Figure Lengend Snippet:

Article Snippet: Detection of secreted human CCL2, IFN-γ, and TNF-α were performed using h.MCP-1 ELISA kit (BOSTER, Wuhan, China, Lot: EK0441), IFN-γ ELISA kit (BOSTER, Wuhan, China, Lot: EK0373), and TNF-α ELISA kit (Absin, Shanghai, China, Lot: abs551818) according to the manufacturers’ instructions.

Techniques: Sequencing